Construction and identification of a specific expression vector for SV40 large T antigen in gastric parietal cell;
SV40T抗原胃壁細(xì)胞特異性表達(dá)載體的構(gòu)建與鑒定
Expression and sgnificance of T antigen in gastric carcinoma patients;
T抗原在胃癌組織中的表達(dá)及臨床意義
OBJECTIVE To design and construct eukaryocyte expression vector of SV40 virus large T antigen and induce its targeted expression in eukaryocyte.
目的設(shè)計、構(gòu)建SV40病毒T抗原真核表達(dá)載體,并導(dǎo)入真核細(xì)胞進(jìn)行表達(dá)鑒定。
Methods We investigated the presence of SV40 T antigen genome in 195 blood specimens from wild monkeys(71 from Nin Lang county,60 from Jin Dong county in Yunnan province) and 64 from caged monkeys by means of the polymerae chain reaction(PCR) followed by sequencing.
目的建立猴外周血單核細(xì)胞SV40 DNA的PCR檢測方法,對獼猴SV40 T抗原基因進(jìn)行檢測。