To establish the technique of RT nested PCR for diagnosis of hemorrhagic fever with renal syndrome(HFRS), and typing of the amplified product was carried out by restriction enzyme digestion.
為建立檢測(cè)腎綜合征出血熱 (HFRS)患者血清中漢坦病毒 (HV)基因的RT nestedPCR方法 ,并進(jìn)一步進(jìn)行限制性酶切分型 ,設(shè)計(jì)并合成互補(bǔ)于HVS基因片段的引物 ,對(duì) 78例HFRS患者血清進(jìn)行RT nestedPCR檢測(cè) ,并對(duì)PCR產(chǎn)物進(jìn)行酶切分析。
complete digestion
完全消化[多指限制酶切反應(yīng)]
Restriction Enzyme Mediated Integration, a New Strategy for Cloning New Genes
限制酶介導(dǎo)整合技術(shù):一種克隆新基因的新策略
Study on Limited Enzymatic Hydrolysis of Soy Protein Concentrate and Soy Protein Isolate and Modification in Functional Properties;
大豆?jié)饪s蛋白和分離蛋白限制酶解及功能性變化
circular restriction map
環(huán)形限制（酶切）圖（譜）
Restriction enzymes and reverse transcriptase are keys to gene engineering.
限制性酶和逆轉(zhuǎn)錄酶成為基因工程的重要手段.
Studies on the Active Groups of Pst I Restriction Endonuclease
限制性核酸內(nèi)切酶PstⅠ活性基團(tuán)的研究
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
方法載體的構(gòu)建采用限制性內(nèi)切酶酶切、4DNA連接酶連接等方法。
Research of Reaction Mechanism and Kinetics Analysis for the FokI Restriction Endonuclease;
限制性內(nèi)切酶FokI作用機(jī)制研究及動(dòng)力學(xué)分析
Secondly, special enzymes called extinction enzymes are used to cut the DNA at specific places.
第二，利用被叫做限制性內(nèi)切酶的特殊的酶在特定地方切斷DNA。
The distribution of polymorphism at restriction enzyme site-48 A/ G was analyzed with the method of polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP).
用聚合酶鏈反應(yīng)-限制性片段長(zhǎng)度多態(tài)性方法,分析限制性酶切位點(diǎn)-48A/的多態(tài)分布。
Guangzhou Enzyme Products Factory
廣州酶制品廠/廣州市華琪生物科技有限公司
Partially hydrolysis of de-gossypol cottonseed protein with trypsinase
胰蛋白酶限制性水解脫酚棉籽蛋白的研究
Effects of limited proteolysis on functional properties of soy 11S glycinin
限制性酶解對(duì)大豆11S球蛋白功能特性的影響
Development and Clinical Application of SARS Virus cDNA Restriction Endoenzyme Analysis Kit;
SARS病毒cDNA限制性內(nèi)切酶檢測(cè)試劑盒的研制和臨床應(yīng)用研究
New phages can develop by acquiring restriction enzymes from plasmids.
通過(guò)從質(zhì)粒獲得的限制性酶,新的噬菌體能夠生長(zhǎng)。
Large DNA molecules are first dissected with restriction enzymes to produce specific fragments.
首先用限制性內(nèi)切酶將大的DNA分子切斷，產(chǎn)生出特殊的片段。
It enables a specific gene to be located on a particular restriction enzyme fragment.
它就能使專一的基因被定位于特定的限制性內(nèi)切酶切成的片段上。
Construction of Protoplast-Based Transformation System of Fungal Biocontrol Agent Paecilomyces Fumosoroseus with Restriction Enzyme-Mediated Integration (REMI);
限制性內(nèi)切酶介導(dǎo)的玫煙色擬青霉原生質(zhì)轉(zhuǎn)化體系構(gòu)建